Article
Transcription termination and 3'-End processing of the spliced leader RNA in kinetoplastids.
Molecular and cellular biology - 1 Feb 1999
Sturm N R, Yu M C, Campbell D A
Abstract excerpt
Addition of a 39-nucleotide (nt) spliced leader (SL) by trans splicing is a basic requirement for all trypanosome nuclear mRNAs. The SL RNA in Leishmania tarentolae is a 96-nt precursor transcript synthesized by a polymerase that resembles polymerase II most closely. To analyze SL RNA genesis, we mutated SL RNA intron structures and sequence elements: stem-loops II and III, the Sm-binding site, and the downstream...
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