Article
Use of the A. victoria green fluorescent protein to study protein dynamics in vivo.
Current protocols in molecular biology - 1 May 2001
Kahana J A, Silver P A
Abstract excerpt
Fluorescent molecules serve as valuable tools for the detection of a variety of biochemical phenomena. Such reagents have been employed for protein localization, quantitation of gene expression, detection of nucleic acids, cell sorting, and determination of chemical concentrations. Although fluorescence is a useful tool for detecting molecules within cells, its application in vivo has heretofore been limited. The...
Topics
- Aequorin
- Animals
- DNA, Complementary
- Genes, Reporter
- Genetic Vectors
- Green Fluorescent Proteins
- Luminescence
- Mutation
- Recombinant Proteins
- Saccharomyces cerevisiae
