Article
Analysis of pCU1 replication origins: dependence of oriS on the plasmid-encoded replication initiation protein RepA.
Plasmid - 1 Mar 2003
Zoueva O P, Iyer V N, Matula T I, Kozlowski M
Abstract excerpt
The broad-host-range replicon of the plasmid pCU1 has three origins of vegetative replication called oriB, oriS, and oriV. In the multi-origin replicon, individual origins can distinguish among replication factors provided by the host. It has been found that during replication in Escherichia coli polA(-) host, oriS was the only active origin of a mutant pCU1 derivative bearing a mutation in the gene encoding...
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