Article
High-throughput mutation detection method to scan BRCA1 and BRCA2 based on heteroduplex analysis by capillary array electrophoresis.
Clinical chemistry - 1 Feb 2004
Esteban-Cardeñosa Eva, Duran Mercedes, Infante Mar, Velasco Eladio, Miner Cristina
Abstract excerpt
BACKGROUND: Scanning for mutations in BRCA1 and BRCA2 in a large number of samples is hampered by the large sizes of these genes and the scattering of mutations throughout their coding sequences. Automated capillary electrophoresis has been shown to be a powerful system to detect mutations by either single-strand conformation polymorphism or heteroduplex analysis (HA). METHODS: We investigated the adaptation of...
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