Article
Temperature-sensitive mutants with lesions in the vaccinia virus F10 kinase undergo arrest at the earliest stage of virion morphogenesis.
Journal of virology - 1 Oct 1995
Traktman P, Caligiuri A, Jesty S A, Liu K, Sankar U
Abstract excerpt
Vaccinia virus encodes two protein kinases; the B1 kinase is expressed early and appears to play a role during DNA replication, whereas the F10 kinase is expressed late and is encapsidated in virions. Here we report that the F10 kinase gene is the locus affected in a complementation group of temperature-sensitive mutants composed of ts15, ts28, ts54, and ts61. Although these mutants have a biochemically normal...
Topics
- Animals
- Cell Line
- Gene Expression
- Genes, Viral
- Genetic Complementation Test
- Microscopy, Electron
- Morphogenesis
- Mutation
- Open Reading Frames
- Phenotype
- Polymerase Chain Reaction