Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Successful amplification of extremely GC-rich promoter regions using a novel 'slowdown PCR' technique.

Pharmacogenetics - 1 Dec 2003

Bachmann Hagen S, Siffert Winfried, Frey Ulrich H

Abstract excerpt

OBJECTIVES: PCR has become a routine technique in DNA genotyping for diagnostic or pharmacogenetics purposes. Promoter regions of genes are in the main focus for detecting novel regulatory single nucleotide polymorphisms (rSNPs). However, due to very high GC content PCR setup procedures can be very time consuming and not infrequently amplification of the regions of interest fail. METHODS: We developed a novel...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to DNA Primers
Share to BRAF gene

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to GNAQ gene

Share to Polymerase Chain Reaction

Share to Polymorphism, Single Nucleotide

Share to Promoter Regions, Genetic

Source

Identifiers

PMID: 14646694
DOI: 10.1097/00008571-200312000-00006

Genes

BRAF
GNAQ

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Successful amplification of extremely GC-rich promoter regions using a novel 'slowdown PCR' technique.PMID 14646694Read ↗

Replacing PCR with COLD-PCR enriches variant DNA sequences and redefines the sensitivity of genetic testing.Semantic proximity Read ↗

COLD-PCR: a new platform for highly improved mutation detection in cancer and genetic testing.Semantic proximity Read ↗

Highly Sensitive Enrichment of Low-Frequency Variants by Hairpin Competition Amplification.Semantic proximity Read ↗

Quasi-digital PCR: Enrichment and quantification of rare DNA variants.Semantic proximity Read ↗

Snapback primer genotyping of the Gilbert syndrome UGT1A1 (TA)(n) promoter polymorphism by high-resolution melting.Semantic proximity Read ↗

Single-step identification of all length polymorphisms in the UGT1A1 gene promoter.Semantic proximity Read ↗

COLD-PCR: improving the sensitivity of molecular diagnostics assays.Semantic proximity Read ↗

Allele-specific, non-extendable primer blocker PCR (AS-NEPB-PCR) for DNA mutation detection in cancer.Semantic proximity Read ↗

Exploring Taq polymerase induced mutations in part of BRAF exon 15 by sequencing and mutation enrichment.Semantic proximity Read ↗

Intrinsic deoxyguanosine quenching of fluorescein-labeled hybridization probes: a simple method for real-time PCR detection and genotyping.Semantic proximity Read ↗

Quantification of single nucleotide polymorphisms: a novel method that combines primer extension assay and capillary electrophoresis.Semantic proximity Read ↗

Sequence-Specific Primer Polymerase Chain Reaction Genotyping of the Human TLR9 Promoter Polymorphism rs187084 (-1486T>C).Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.