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Article

Disruption of the PU.1 gene in chicken B lymphoma DT40 cells and its effect on reported target gene expression.

Gene - 11 Dec 2003

Matsudo Hiroki, Otsuka Akira, Ozawa Yukiko, Ono Masao

Abstract excerpt

Using avian B lymphoma-derived DT40 cells, we disrupted a gene encoding the transcription factor PU.1. The mutant mRNA codes for a protein incapable of functioning as a transcription factor because of the deletion of the protein's DNA-binding domain. The absence of a functional PU.1 protein in the mutant cells was confirmed by Western blotting and electrophoretic mobility shift assay, thereby demonstrating that...

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Identifiers

PMID: 14644508
DOI: 10.1016/j.gene.2003.08.021

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Disruption of the PU.1 gene in chicken B lymphoma DT40 cells and its effect on reported target gene expression.PMID 14644508Read ↗

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