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Article

Mutagenesis of human DNA polymerase lambda: essential roles of Tyr505 and Phe506 for both DNA polymerase and terminal transferase activities.

Nucleic acids research - 1 Dec 2003

Shevelev Igor, Blanca Giuseppina, Villani Giuseppe, Ramadan Kristijan, Spadari Silvio, Hübscher Ulrich, Maga Giovanni

Abstract excerpt

DNA polymerase (pol) lambda is homologous to pol beta and has intrinsic polymerase and terminal transferase activities. However, nothing is known about the amino acid residues involved in these activities. In order to precisely define the nucleotide-binding site of human pol lambda, we have mutagenised two amino acids, Tyr505 and the neighbouring Phe506, which were predicted by structural homology modelling to...

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Identifiers

PMID: 14627824
DOI: 10.1093/nar/gkg896
PMCID: PMC290264

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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