Article
Inactivation of the murine N-ras gene by gene targeting.
Oncogene - 1 Dec 1992
Cases S, Dautry F
Abstract excerpt
The mammalian ras genes have been implicated in the genesis of a wide variety of tumors. Although it is likely that they play an essential role in signal transduction, their specific function is still unknown. To initiate a genetic analysis of the ras genes in mammals we inactivated the N-ras gene in murine embryonic stem cells by gene targeting. The frequency of integration at the N-ras locus of our targeting...
Topics
- Alleles
- Animals
- Base Sequence
- Blotting, Southern
- Chimera
- Cloning, Molecular
- DNA
- Embryo, Mammalian
- Gene Expression Regulation
- Genes, ras
- Genetic Vectors
- Mice