Article
Target DNA bending is an important specificity determinant in target site selection in Tn10 transposition.
Journal of molecular biology - 4 Jul 2003
Pribil Patrick A, Haniford David B
Abstract excerpt
The bacterial transposon Tn10 inserts preferentially into specific DNA sequences. DNA footprinting and interference studies have revealed that the Tn10-encoded transposase protein contacts a large stretch of target DNA ( approximately 24 bp) and that the target DNA structure is deformed upon incorporation into the transpososome. Target DNA deformation might contribute significantly to target site selection and...
Topics
- Base Sequence
- Binding Sites
- Calcium
- DNA Footprinting
- DNA Transposable Elements
- DNA, Bacterial
- Exodeoxyribonucleases
- Models, Biological
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation