Article
Development and characterization of a stable epithelial cell line from Muta Mouse lung.
Environmental and molecular mutagenesis - 1 Jan 2003
White Paul A, Douglas George R, Gingerich John, Parfett Craig, Shwed Phil, Seligy Vern, Soper Lynda, Berndt Lynn, Bayley Janet, Wagner Shelley, Pound Kathleen, Blakey David
Abstract excerpt
We have isolated and characterized a stable epithelial cell line from Muta Mouse lung that is a suitable complement to the in vivo assay system. The cells are contact inhibited, forming a flat monolayer, and retain several epithelial/pulmonary characteristics. The genome is stable across more than 50 generations, with a modal chromosome number of 78. Spontaneous rates of micronuclei (19.2 +/- 1.4 per 1,000),...
Topics
- Animals
- Epithelial Cells
- Lac Operon
- Lung
- Mice
- Mice, Mutant Strains
- Microscopy, Fluorescence
- Mutation
- Transgenes