Article
Induction of lacZ mutations in MutaMouse primary hepatocytes.
Environmental and molecular mutagenesis - 1 May 2010
Chen Guosheng, Gingerich John, Soper Lynda, Douglas George R, White Paul A
Abstract excerpt
We have developed an in vitro mutation assay using primary hepatocytes from the transgenic MutaMouse. Primary hepatocytes were isolated using a two-step perfusion method with purification by Percoll, cultured, and treated with benzo[a]pyrene (BaP), 2-amino-1-methyl-6-phenyl- imidazo[4,5-b]pyridine (PhIP), 3-nitrobenzoanthrone (3-NBA), and cigarette smoke condensate (CSC). The mean lacZ mutant frequency (MF) for...
Topics
- Aminopyridines
- Animals
- Benzo(a)pyrene
- Cells, Cultured
- Cytochrome P-450 Enzyme System
- Hepatocytes
- Imidazoles
- Lac Operon
- Mice
- Mice, Mutant Strains
- Mutagenicity Tests
- Mutagens
- Mutation