Article
Binding of TFIIB to RNA polymerase II: Mapping the binding site for the TFIIB zinc ribbon domain within the preinitiation complex.
Molecular cell - 1 Aug 2003
Chen Hung-Ta, Hahn Steven
Abstract excerpt
RNA polymerase II (Pol II) is recruited to promoters by interaction with general transcription factors. The zinc ribbon domain of the general factor TFIIB is essential for Pol II recruitment. Site-specific photocrosslinking and directed hydroxyl radical probing were used to map the location of the TFIIB zinc ribbon domain on Pol II within the transcription preinitiation complex (PIC). These results, along with...
Topics
- Amino Acid Sequence
- Binding Sites
- Cell Nucleus
- Cross-Linking Reagents
- DNA Mutational Analysis
- Epitopes
- Fungal Proteins
- Hydroxyl Radical
- Light
- Models, Genetic
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Peptides
- Precipitin Tests