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Article

A zinc-binding site in the largest subunit of DNA-dependent RNA polymerase is involved in enzyme assembly.

Genes & development - 15 Sept 1999

Markov D, Naryshkina T, Mustaev A, Severinov K

Abstract excerpt

All multisubunit DNA-dependent RNA polymerases (RNAP) are zinc metalloenzymes, and at least two zinc atoms are present per enzyme molecule. RNAP residues involved in zinc binding and the functional role of zinc ions in the transcription mechanism or RNAP structure are unknown. Here, we locate four cysteine residues in the Escherichia coli RNAP largest subunit, beta', that coordinate one of the two zinc ions...

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Identifiers

PMID: 10500100
DOI: 10.1101/gad.13.18.2439
PMCID: PMC317019

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Source receipt: pubmed, retrieved 7 Sept 2026.

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