In vitro analysis of mutant LexA proteins with an increased rate of specific cleavage.

doi:10.1016/0022-2836(92)90829-9

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Article

In vitro analysis of mutant LexA proteins with an increased rate of specific cleavage.

Journal of molecular biology - 20 Nov 1992

Roland K L, Smith M H, Rupley J A, Little J W

Abstract excerpt

Specific cleavage of LexA repressor plays a crucial role in the SOS response of Escherichia coli. In vivo, cleavage requires an activated form of RecA protein. However, previous work has shown that the mechanism of cleavage is unusual, in that the chemistry of cleavage is probably carried out by...

Topics

Bacterial Proteins
Escherichia coli
Hydrogen-Ion Concentration
Isoflurophate
Kinetics
Mutation
Plasmids
Protein Conformation
Rec A Recombinases
Repressor Proteins
Restriction Mapping
Serine Endopeptidases

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