Article
Mutant LexA proteins with an increased rate of in vivo cleavage.
Proceedings of the National Academy of Sciences of the United States of America - 15 Aug 1991
Smith M H, Cavenagh M M, Little J W
Abstract excerpt
LexA repressor of Escherichia coli is inactivated by a specific cleavage reaction that requires activated RecA protein in vivo. This cleavage reaction can proceed in vitro in the presence of activated RecA or as an intramolecular RecA-independent reaction, termed autodigestion, that is stimulated by alkaline pH. Here we describe a set of LexA mutant proteins that undergo a greatly increased rate of specific...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Escherichia coli
- Genotype
- Kinetics
- Mutagenesis, Site-Directed
- Plasmids
- Repressor Proteins
- Serine Endopeptidases
- beta-Galactosidase