Article
The endogenous Mus81-Eme1 complex resolves Holliday junctions by a nick and counternick mechanism.
Molecular cell - 1 Sept 2003
Gaillard Pierre-Henri L, Noguchi Eishi, Shanahan Paul, Russell Paul
Abstract excerpt
Functional studies strongly suggest that the Mus81-Eme1 complex resolves Holliday junctions (HJs) in fission yeast, but in vitro it preferentially cleaves flexible three-way branched structures that model replication forks or 3' flaps. Here we report that a nicked HJ is the preferred substrate of endogenous and recombinant Mus81-Eme1. Cleavage occurs specifically on the strand that opposes the nick, resulting in...
Topics
- Cells, Cultured
- DNA
- DNA Polymerase I
- DNA Replication
- DNA-Binding Proteins
- Endonucleases
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Fungal
- Macromolecular Substances
- Mutation
- Nucleic Acid Conformation