Article
Substrate preference of Gen endonucleases highlights the importance of branched structures as DNA damage repair intermediates.
Nucleic acids research - 19 May 2017
Bellendir Stephanie P, Rognstad Danielle J, Morris Lydia P, Zapotoczny Grzegorz, Walton William G, Redinbo Matthew R, Ramsden Dale A, Sekelsky Jeff, Erie Dorothy A
Abstract excerpt
Human GEN1 and yeast Yen1 are endonucleases with the ability to cleave Holliday junctions (HJs), which are proposed intermediates in recombination. In vivo, GEN1 and Yen1 function secondarily to Mus81, which has weak activity on intact HJs. We show that the genetic relationship is reversed in Drosophila, with Gen mutants having more severe defects than mus81 mutants. In vitro, DmGen, like HsGEN1, efficiently...
Topics
- Animals
- Base Sequence
- Cytoplasm
- DNA Damage
- DNA Repair
- DNA Replication
- DNA, Cruciform
- DNA-Binding Proteins
- Drosophila Proteins
- Drosophila melanogaster