Article
A mutation in the tRNA nucleotidyltransferase gene promotes stabilization of mRNAs in Saccharomyces cerevisiae.
Molecular and cellular biology - 1 Dec 1992
Peltz S W, Donahue J L, Jacobson A
Abstract excerpt
To identify trans-acting factors involved in mRNA decay in the yeast Saccharomyces cerevisiae, we have begun to characterize conditional lethal mutants that affect mRNA steady-state levels. A screen of a collection of temperature-sensitive mutants identified ts352, a mutant that accumulated moderately stable and unstable mRNAs after a shift from 23 to 37 degrees C (M. Aebi, G. Kirchner, J.-Y. Chen, U....
Topics
- Cycloheximide
- Fungal Proteins
- Kinetics
- Mutation
- Polyribosomes
- Protein Biosynthesis
- RNA Nucleotidyltransferases
- RNA, Fungal
- RNA, Messenger
- RNA, Transfer
- Saccharomyces cerevisiae