Article
Isolation of a temperature-sensitive mutant with an altered tRNA nucleotidyltransferase and cloning of the gene encoding tRNA nucleotidyltransferase in the yeast Saccharomyces cerevisiae.
The Journal of biological chemistry - 25 Sept 1990
Aebi M, Kirchner G, Chen J Y, Vijayraghavan U, Jacobson A, Martin N C, Abelson J
Abstract excerpt
We have isolated a yeast mutant, ts352, that is temperature-sensitive for growth. The mutation has a general effect on mRNA metabolism and a specific effect on tRNA biosynthesis. Cells shifted to the nonpermissive temperature accumulate tRNAs that are shorter than mature tRNAs. The increased abil...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Genes, Fungal
- Molecular Sequence Data
- Mutation
- Plasmids
- RNA Nucleotidyltransferases
- Restriction Mapping
- Saccharomyces cerevisiae
- Sequence Homology, Nucleic Acid
- Temperature