Article
An improved fluorometric assay for dosimetry of benzo(a)pyrene diol-epoxide-DNA adducts in smokers' lung: comparisons with total bulky adducts and aryl hydrocarbon hydroxylase activity.
Cancer research - 15 Nov 1992
Alexandrov K, Rojas M, Geneste O, Castegnaro M, Camus A M, Petruzzelli S, Giuntini C, Bartsch H
Abstract excerpt
An improved high-performance liquid chromatography/fluorometric assay has been established to quantitate the benzo(a)pyrene (BP) tetrols released after acid hydrolysis of lung DNA from lung cancer patients, so that the formation of benzo(a)pyrene diol-epoxide-DNA adducts can be measured. The r-7,c-10,t-8,t-9-tetrahydroxy-7,8,9,10-tetrahydro-BP isolated by high-performance liquid chromatography was determined by...
Topics
- 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
- Adult
- Aged
- Animals
- Aryl Hydrocarbon Hydroxylases
- Benzo(a)pyrene
- Cattle
- Chromatography, High Pressure Liquid
- DNA
- DNA Adducts