Article
Evidence that multiple myeloma Ig heavy chain VDJ genes contain somatic mutations but show no intraclonal variation.
Blood - 1 Nov 1992
Bakkus M H, Heirman C, Van Riet I, Van Camp B, Thielemans K
Abstract excerpt
To investigate whether somatic hypermutation occurs in multiple myeloma (MM) Ig VH region genes, we have cloned and sequenced the expressed VH genes from five cases of MM. The sequences were obtained after polymerase chain reaction (PCR) on total RNA isolated from the bone marrow, using 5' VH fam...
Topics
- Adult
- Amino Acid Sequence
- Base Sequence
- Bone Marrow
- DNA, Neoplasm
- Female
- Genes, Immunoglobulin
- Humans
- Immunoglobulin Heavy Chains
- Immunoglobulin Joining Region
- Immunoglobulin Variable Region
- Male
- Middle Aged
- Molecular Sequence Data
- Multigene Family
- Multiple Myeloma
- Mutation
- Oligodeoxyribonucleotides