Article
Myeloma Ig heavy chain V region sequences reveal prior antigenic selection and marked somatic mutation but no intraclonal diversity.
Journal of immunology (Baltimore, Md. : 1950) - 1 Sept 1995
Vescio R A, Cao J, Hong C H, Lee J C, Wu C H, Der Danielian M, Wu V, Newman R, Lichtenstein A K, Berenson J R
Abstract excerpt
The lg VH region sequence in 48 patients with multiple myeloma (MM) was analyzed to characterize the malignant cell of origin. The sequences were obtained after amplification of bone marrow cDNA by using VH family-specific and CH primers, then compared with either directly sequenced patient germ-line or published VH gen sequences to assay for somatic mutation. Because somatic hypermutation of the VH gene occurs...
Topics
- Amino Acid Sequence
- Antibody Diversity
- Antigen Presentation
- Base Sequence
- Clone Cells
- Humans
- Immunoglobulin Heavy Chains
- Immunoglobulin Variable Region
- Molecular Sequence Data
- Multiple Myeloma