Use of DNA purified in situ from cells embedded in agarose plugs for the molecular analysis of tk-/- mutants recovered in the L5178Y tk+/- 3.7.2C mutagen assay system.

doi:10.1016/0165-7992(90)90026-g

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Article

Use of DNA purified in situ from cells embedded in agarose plugs for the molecular analysis of tk-/- mutants recovered in the L5178Y tk+/- 3.7.2C mutagen assay system.

Mutation research - 1 Sept 1990

Applegate M, Juhn G, Liechty M, Moore M, Hozier J

Abstract excerpt

We have reported that tk-/- mutants recovered in the mouse L5178Y tk+/- 3.7.2C mutagen assay have often lost the tk+ allele. Allele loss in the tk-/- mutants is documented on Southern blots as the absence of a 6.3-kb Nco I fragment seen in both tk+/+ and tk+/- cell DNAs. For the routine screening...

Topics

Alleles
Animals
DNA
DNA Mutational Analysis
DNA, Neoplasm
Leukemia L5178
Mice
Mutation
Thymidine Kinase

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