Article
Mutant isolation of mouse DNA topoisomerase II alpha in yeast.
Nucleic acids research - 11 Oct 1994
Adachi N, Ikeda H, Kikuchi A
Abstract excerpt
For characterizing in vivo functions of a mammalian protein, it is informative to obtain conditional mutations and apply them to the mouse genetic system. However, the isolation of conditional mutations has been quite difficult in cultured cells. We report here that functional expression of a het...
Topics
- Amino Acid Sequence
- Animals
- Antigens, Neoplasm
- Base Sequence
- DNA Topoisomerases, Type II
- DNA-Binding Proteins
- Galactose
- Gene Expression
- Gene Transfer Techniques
- Hot Temperature
- Isoenzymes
- Mice
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Plasmids
- Poly-ADP-Ribose Binding Proteins
- Saccharomyces cerevisiae