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Article

Development of a novel mouse tk+/- embryonic stem cell line for use in mutagenicity studies.

Environmental and molecular mutagenesis - 1 Jan 1996

Dobrovolsky V N, Casciano D A, Heflich R H

Abstract excerpt

A tk+/- mouse embryonic stem (ES) cell line, designated 1G2, has been created in which one allele of the thymidine kinase (tk) gene was inactivated by targeted homologous recombination. This line is an analog of the mouse lymphoma tk+/- L5178Y cell line, which is used widely to assess the mutagenicity of chemical agents. Treatment of 1G2 cells with the alkylating agent N-ethyl-N-nitrosourea (ENU) resulted in a...

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Source

Identifiers

PMID: 8991081
DOI: 10.1002/(SICI)1098-2280(1996)28:4<483::AID-EM26>3.0.CO;2-A

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