Article
Development of a novel mouse tk+/- embryonic stem cell line for use in mutagenicity studies.
Environmental and molecular mutagenesis - 1 Jan 1996
Dobrovolsky V N, Casciano D A, Heflich R H
Abstract excerpt
A tk+/- mouse embryonic stem (ES) cell line, designated 1G2, has been created in which one allele of the thymidine kinase (tk) gene was inactivated by targeted homologous recombination. This line is an analog of the mouse lymphoma tk+/- L5178Y cell line, which is used widely to assess the mutagen...
Topics
- Animals
- Antimetabolites
- Blotting, Southern
- Cell Line
- Cloning, Molecular
- Drug Resistance
- Embryo, Mammalian
- Ethylnitrosourea
- Genetic Vectors
- Mice
- Mutagenicity Tests
- Mutagens
- Mutation
- Polymerase Chain Reaction
- Recombination, Genetic
- Stem Cells
- Thymidine Kinase
- Trifluridine