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Article

DNA typing of the HLA-A gene: population study and identification of four new alleles in Japanese.

Tissue antigens - 1 Feb 1996

Date Y, Kimura A, Kato H, Sasazuki T

Abstract excerpt

With the use of polymerase chain reaction (PCR) and sequence-specific oligonucleotide probe (SSOP), we established a DNA typing method of the HLA-A locus. A pair of primers to amplify the highly polymorphic region of HLA-A gene including exon 2 and exon 3 was designed and the amplified DNAs were hybridized with 91 types of 32P labeled SSOPs. This method allowed discrimination of all known HLA-A alleles except for...

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PMID: 8851721
DOI: 10.1111/j.1399-0039.1996.tb02520.x

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