Article
DNA typing of the HLA-A gene: population study and identification of four new alleles in Japanese.
Tissue antigens - 1 Feb 1996
Date Y, Kimura A, Kato H, Sasazuki T
Abstract excerpt
With the use of polymerase chain reaction (PCR) and sequence-specific oligonucleotide probe (SSOP), we established a DNA typing method of the HLA-A locus. A pair of primers to amplify the highly polymorphic region of HLA-A gene including exon 2 and exon 3 was designed and the amplified DNAs were...
Topics
- Alleles
- B-Lymphocytes
- Base Sequence
- Cell Line
- DNA
- DNA Primers
- Exons
- HLA-A Antigens
- Humans
- Japan
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- Polymerase Chain Reaction
- Population Surveillance
- Sequence Analysis