Simplified PCR-SSO procedure for DQA and DQB typing. Simultaneous hybridization of two probes to one membrane.

doi:10.1016/0198-8859(96)00013-4

Back to search

Article

Simplified PCR-SSO procedure for DQA and DQB typing. Simultaneous hybridization of two probes to one membrane.

Human immunology - 1 Apr 1996

Chen D F, Endres W, Meyer S A, Stangel W

Abstract excerpt

To simplify DQA and DQB oligotyping, we applied our improved PCR-SSO procedure for DR typing. We used 12 oligonucleotide probes for DQA typing and 18 for DQB typing. Oligonucleotide probes that require the same hybridization and stringent washing conditions were selected as pairs for simultaneous...

Topics

Genotype
HLA-DQ Antigens
HLA-DQ alpha-Chains
HLA-DQ beta-Chains
Humans
Membranes, Artificial
Nucleic Acid Hybridization
Oligonucleotide Probes
Polymerase Chain Reaction

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.