Article
A novel accurate amplification created restriction site method for determination of the wild type and the precore mutant hepatitis B virus variants.
Journal of virological methods - 1 Jul 2005
Amini-Bavil-Olyaee Samad, Sarrami-Forooshani Ramin, Adeli Ahmad, Mahboudi Fereidoun, Sabahi Farzaneh, Nafisi Houman, Zali Mohammad Reza, Azizi Mohammad
Abstract excerpt
The most commonly occurring hepatitis B virus (HBV) mutation is the G to A mutation at nucleotide 1896 in the precore region. The aim of this study was to develop a novel accurate amplification created restriction site (ACRS) method for determination of the TGG wild type and the TAG precore mutant HBV variants. Two conserved and consensus specific and diagnostic primers introducing BstXI and XagI cleavage sites...
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