Elongation by Escherichia coli RNA polymerase is blocked in vitro by a site-specific DNA binding protein.

Abstract excerpt

As a means of determining how elongating RNA polymerase responds to a protein in its path, transcription has been carried out in vitro with the purified Escherichia coli enzyme on templates associated with a sequence-specific DNA binding protein. The major RNA species generated is the length expected from RNA polymerase which has transcribed to the position of the bound protein and is unable to elongate further....

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