Article
Elongation by Escherichia coli RNA polymerase is blocked in vitro by a site-specific DNA binding protein.
The Journal of biological chemistry - 15 Jun 1990
Pavco P A, Steege D A
Abstract excerpt
As a means of determining how elongating RNA polymerase responds to a protein in its path, transcription has been carried out in vitro with the purified Escherichia coli enzyme on templates associated with a sequence-specific DNA binding protein. The major RNA species generated is the length expe...
Topics
- Bacteriophage lambda
- Base Sequence
- Binding Sites
- DNA-Binding Proteins
- DNA-Directed RNA Polymerases
- Deoxyribonuclease EcoRI
- Escherichia coli
- Glutamine
- Glycine
- Models, Genetic
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- RNA, Bacterial
- Restriction Mapping
- Templates, Genetic
- Transcription, Genetic