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A colony color assay for Saccharomyces cerevisiae mutants defective in kinetochore structure and function.

Genetics - 1 Sept 1992

Périer F, Carbon J

Abstract excerpt

We have designed a colony color assay for monitoring centromere DNA-protein interactions in yeast (Saccharomyces cerevisiae). The assay is based on the ability of centromere DNA sequences to block (in cis) transcription initiated from a hybrid CEN-GAL1 promoter. Using a IacZ reporter gene under control of the CEN-GAL1 promoter, we screened colonies derived from mutagenized cells for a blue color phenotype...

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Identifiers

PMID: 1398062
DOI: 10.1093/genetics/132.1.39
PMCID: PMC1205128

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