Article
A colony color assay for Saccharomyces cerevisiae mutants defective in kinetochore structure and function.
Genetics - 1 Sept 1992
Périer F, Carbon J
Abstract excerpt
We have designed a colony color assay for monitoring centromere DNA-protein interactions in yeast (Saccharomyces cerevisiae). The assay is based on the ability of centromere DNA sequences to block (in cis) transcription initiated from a hybrid CEN-GAL1 promoter. Using a IacZ reporter gene under c...
Topics
- Acriflavine
- Base Sequence
- Centromere
- Colorimetry
- DNA, Fungal
- DNA-Binding Proteins
- Flow Cytometry
- Fungal Proteins
- Genes, Dominant
- Genes, Fungal
- Genes, Recessive
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Plasmids
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins
- Transcription Factors