Article
Analysis of centromere function in Saccharomyces cerevisiae using synthetic centromere mutants.
Chromosoma - 1 Dec 1991
Murphy M R, Fowlkes D M, Fitzgerald-Hayes M
Abstract excerpt
We constructed Saccharomyces cerevisiae centromere DNA mutants by annealing and ligating synthetic oligonucleotides, a novel approach to centromere DNA mutagenesis that allowed us to change only one structural parameter at a time. Using this method, we confirmed that CDE I, II, and III alone are sufficient for centromere function and that A + T-rich sequences in CDE II play important roles in mitosis and meiosis....
Topics
- Base Sequence
- Centromere
- Chromosomes, Fungal
- Consensus Sequence
- DNA, Fungal
- Electrophoresis, Polyacrylamide Gel
- Molecular Sequence Data
- Mutation
- Saccharomyces cerevisiae