Article
Mutations affecting RNA-DNA hybrid formation of the ColE1 replication primer RNA. Restoration of RNA I sensitivity to a copy-number mutant by second-site mutations.
Journal of molecular biology - 20 Aug 1992
Fitzwater T, Yang Y L, Zhang X Y, Polisky B
Abstract excerpt
Certain high copy-number mutants of the ColE1 plasmid produce a primer RNA that, unlike the wild-type, is resistant to inhibition by the plasmid-encoded replication inhibitor RNA I. We show that this resistance is associated with the ability of mutant primer RNA to hybridize to the DNA template s...
Topics
- Bacteriocin Plasmids
- Base Sequence
- DNA Replication
- Escherichia coli
- Molecular Sequence Data
- Multigene Family
- Mutation
- Nucleic Acid Conformation
- Nucleic Acid Hybridization
- Oligonucleotide Probes
- RNA, Bacterial
- RNA, Messenger
- Transcription, Genetic