Article
Suppression of ColE1 high-copy-number mutants by mutations in the polA gene of Escherichia coli.
Journal of bacteriology - 1 Jan 1993
Yang Y L, Polisky B
Abstract excerpt
We isolated three Escherichia coli suppressor strains that reduce the copy number of a mutant ColE1 high-copy-number plasmid. These mutations lower the copy number of the mutant plasmid in vivo up to 15-fold; the wild-type plasmid copy number is reduced by two- to threefold. The suppressor strain...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Colicins
- Crosses, Genetic
- DNA Polymerase I
- Escherichia coli
- Genes, Bacterial
- Genetic Complementation Test
- Kinetics
- Molecular Sequence Data
- Mutagenesis
- Mutagenesis, Site-Directed
- Nitrosoguanidines
- Oligodeoxyribonucleotides
- Phenotype
- Plasmids
- Protein Structure, Secondary