Article
Retrotransposon Ty1 RNA contains a 5'-terminal long-range pseudoknot required for efficient reverse transcription.
RNA (New York, N.Y.) - 1 Mar 2013
Huang Qing, Purzycka Katarzyna J, Lusvarghi Sabrina, Li Donghui, Legrice Stuart F J, Boeke Jef D
Abstract excerpt
Ty1 retrotransposon RNA has the potential to fold into a variety of distinct structures, mutation of which affects retrotransposition frequencies. We show here that one potential functional structure is located at the 5' end of the genome and can assume a pseudoknot conformation. Chemoenzymatic probing of wild-type and mutant mini-Ty1 RNAs supports the existence of such a structure, while molecular genetic...
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