Site-specific RNase E cleavage of oligonucleotides and inhibition by stem-loops.

doi:10.1038/374287a0

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Article

Site-specific RNase E cleavage of oligonucleotides and inhibition by stem-loops.

Nature - 16 Mar 1995

McDowall K J, Kaberdin V R, Wu S W, Cohen S N, Lin-Chao S

Abstract excerpt

The enzyme RNase E (ref. 1) cuts RNA at specific sites within single-stranded segments. The role of adjacent regions of secondary structure in such cleavages is controversial. Here we report that 10-13-nucleotide oligomers lacking any stem-loop but containing the RNase E-cleaved sequence of RNA I...

Topics

Base Sequence
Endoribonucleases
Escherichia coli
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
Oligoribonucleotides
RNA, Bacterial
Ribonuclease T1
Substrate Specificity

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