Molecular screening of genetic defects with RNA-SSCP analysis: the PKU and cystinuria model.

doi:10.1006/mcpr.1995.0032

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Article

Molecular screening of genetic defects with RNA-SSCP analysis: the PKU and cystinuria model.

Molecular and cellular probes - 1 Jun 1995

Giannattasio S, Bisceglia L, Lattanzio P, Grifa A, Dianzani I, Gasparini P, Marra E

Abstract excerpt

RNA single-strand conformation polymorphism (rSSCP) is a recently developed method for detecting genetic defects. This technique requires DNA amplification with a polymerase chain reaction making use of one T7 promoter-containing primer. Amplification products are subsequently transcribed in vitr...

Topics

Base Sequence
Cystinuria
DNA, Complementary
Exons
Genetic Testing
Humans
Italy
Molecular Sequence Data
Mutation
Phenylalanine Hydroxylase
Phenylketonurias
Polymorphism, Single-Stranded Conformational
RNA

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