Transformation-defective mutants of polyomavirus middle T antigen associate with phosphatidylinositol 3-kinase (PI 3-kinase) but are unable to maintain wild-type levels of PI 3-kinase products in intact cells.

Abstract excerpt

Middle T antigen (MT) of polyomavirus causes transformation by associating with a number of cellular proteins. The association with and activation of two such proteins, phosphatidylinositol 3-kinase (PI 3-kinase) and pp60c-src, appears to be necessary for transformation by MT. The tyrosine kinase activity of MT-associated pp60c-src is significantly increased when assayed in vitro, and levels of...

Topics

• 1-Phosphatidylinositol 4-Kinase
• Antigens, Polyomavirus Transforming
• Cell Transformation, Viral
• Cells, Cultured
• In Vitro Techniques
• Mutation
• Phosphatidylinositols
• Phosphotransferases
• Polyomavirus
• Proto-Oncogene Proteins pp60(c-src)