Mutation of a cysteine residue in polyomavirus middle T antigen abolishes interactions with protein phosphatase 2A, pp60c-src, and phosphatidylinositol-3 kinase, activation of c-fos expression, and cellular transformation.

doi:10.1128/JVI.67.4.1945-1952.1993

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Mutation of a cysteine residue in polyomavirus middle T antigen abolishes interactions with protein phosphatase 2A, pp60c-src, and phosphatidylinositol-3 kinase, activation of c-fos expression, and cellular transformation.

Journal of virology - 1 Apr 1993

Glenn G M, Eckhart W

Abstract excerpt

Polyomavirus middle T antigen (MT) interacts with several cellular proteins involved in cell proliferation. MT forms complexes with protein phosphatase 2A (PP2A), pp60c-src (and the related kinases c-fyn and c-yes), and phosphatidylinositol-3 kinase. We made a single point mutation in MT, changing a conserved cysteine residue at position 120 to tryptophan, and characterized the biochemical and biological...

Topics

1-Phosphatidylinositol 4-Kinase
3T3 Cells
Amino Acid Sequence
Animals
Antigens, Polyomavirus Transforming
Cell Transformation, Viral
Cloning, Molecular
Cysteine
Gene Expression Regulation, Viral
Genes, fos

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

In Vitro Techniques

Macromolecular Substances

Mice

Molecular Sequence Data

Mutation

Phosphoprotein Phosphatases

Phosphotransferases

Polyomavirus

Source

Open in PubMed Open full text

Identifiers

PMID: 7680388
DOI: 10.1128/JVI.67.4.1945-1952.1993
PMCID: PMC240262

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7680388-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Mutation of a cysteine residue in polyomavirus middle T antigen abolishes interactions with protein phosphatase 2A, pp60c-src, and phosphatidylinositol-3 kinase, activation of c-fos expression, and cellular transformation.PMID 7680388Read ↗

A Transformation-Defective Polyomavirus Middle T Antigen with a Novel Defect in PI3 Kinase Signaling.Semantic proximity Read ↗

pp60c-src binding to polyomavirus middle T-antigen (MT) requires residues 185 to 210 of the MT sequence.Semantic proximity Read ↗

Identification of regions in polyomavirus middle T and small t antigens important for association with protein phosphatase 2A.Semantic proximity Read ↗

Myristylation of pp60c-src is not required for complex formation with polyomavirus middle-T antigen.Semantic proximity Read ↗

The amino terminus of polyomavirus middle T antigen is required for transformation.Semantic proximity Read ↗

Polyoma middle tumor antigen interacts with SHC protein via the NPTY (Asn-Pro-Thr-Tyr) motif in middle tumor antigen.Semantic proximity Read ↗

Signaling from polyomavirus middle T and small T defines different roles for protein phosphatase 2A.Semantic proximity Read ↗

Transformation-defective mutants of polyomavirus middle T antigen associate with phosphatidylinositol 3-kinase (PI 3-kinase) but are unable to maintain wild-type levels of PI 3-kinase products in intact cells.Semantic proximity Read ↗

Domains in middle-T antigen that cooperate in polyomavirus-mediated oncogenic transformation.Semantic proximity Read ↗

Polyomavirus middle-T antigen lacking a membrane anchor sequence accumulates in the nucleus.Semantic proximity Read ↗

Association of Polyomavirus Middle Tumor Antigen with 14-3-3 ProteinsSemantic proximity Read ↗

Transformation and tumorigenic properties of a mutant polyomavirus containing a middle T antigen defective in Shc binding.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.