Article
Nucleotide sequence and PCR-amplification of a polymorphic MboI site in human DNA marker D4S95 linked to the Huntington disease locus.
Clinical genetics - 1 Oct 1992
Nørremølle A, Sørensen S A, Arctander P
Abstract excerpt
The nucleotide sequence of the probe BS674E-D defining the genetic locus D4S95 was assessed, and used for designing PCR-primers amplifying a 175 bp fragment containing a polymorphic MboI site. With the described conditions it is now possible to use PCR followed by digestion for rapid determinatio...
Topics
- Base Sequence
- DNA Probes
- Deoxyribonucleases, Type II Site-Specific
- Genotype
- Humans
- Huntington Disease
- Molecular Sequence Data
- Pedigree
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Sequence Analysis, DNA