Article
Assay by polymerase chain reaction (PCR) of multi-allele polymorphisms in the Huntington's disease region of chromosome 4.
Molecular and cellular probes - 1 Dec 1992
Allitto B A, McClatchey A I, Barnes G, Altherr M, Wasmuth J, Frischauf A M, MacDonald M E, Gusella J
Abstract excerpt
The Huntington's disease-linked D4S115 marker has been converted from a DNA blot assay to a more sensitive and rapid polymerase chain reaction (PCR) assay. PCR amplification of a tandem repeat at D4S115 revealed 7 allelic fragments, ranging in size from approximately 610 to 915 bp, differing in their apparent copy number of a approximately 55 bp core repeat. This repeat unit differs strikingly in sequence from...
Topics
- Alleles
- Base Sequence
- Chromosomes, Human, Pair 4
- Gene Frequency
- Genetic Counseling
- Genetic Markers
- Humans
- Huntington Disease
- Molecular Sequence Data
- Polymerase Chain Reaction