PCR analysis of a three-allelic PvuII--RFLP at D4S127 closely linked to the Huntington disease locus.

doi:10.1111/j.1399-0004.1993.tb03828.x

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Article

PCR analysis of a three-allelic PvuII--RFLP at D4S127 closely linked to the Huntington disease locus.

Clinical genetics - 1 Jun 1993

Willoh R, Zühlke C, Gerdes B, Wiese S, Thies U

Abstract excerpt

The polymorphic probe BJ56PS18 defined the genetic locus D4S127 closely linked to the Huntington disease (HD) gene. BJ56PS18 detects a three-allelic polymorphism after PvuII digestion in Southern blots. For rapid genotyping in HD-families, two different PCR conditions were established.

Topics

Alleles
Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 4
Genetic Linkage
Humans
Huntington Disease
Molecular Sequence Data
Polymerase Chain Reaction
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length

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