Article
Improvement in HLA-DQB typing by PCR-RFLP: introduction of a constant restriction site in one of the primers for digestion control.
Tissue antigens - 1 Aug 1992
Mercier B, Ferec C, Dufosse F, Huart J J
Abstract excerpt
The PCR-RFLP method previously reported by Inoko is a powerful technique for HLA class II typing. The reliability of RFLP interpretation depends on complete digestion by restriction endonucleases using a modified primer with restriction sites as an internal digestion control. The use of restriction enzymes which recognize specific HLA DQB allelic variations makes HLA DQB genotyping possible.
Topics
- Alleles
- Base Sequence
- DNA
- Genotype
- HLA-DQ Antigens
- HLA-DQ beta-Chains
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length