Article
HLA-DQB1 allele typing by a new PCR-RFLP method: correlation with a PCR-SSO method.
Tissue antigens - 1 Sept 1992
Salazar M, Yunis J J, Delgado M B, Bing D, Yunis E J
Abstract excerpt
We have developed a new PCR-RFLP method for HLA-DQB1 typing. This method was easy to follow, requiring only one DQB1 generic amplification and 5 endonucleases to assign 14 out of 15 HLA-DQB1 alleles. In addition, we determined that by using one generic amplification and two enzymes (Sau96 I and Hae III) it was possible to type the generic specificities: DQw2, DQw4, DQw5, DQw6, and DQw7, DQw8-9, providing a...
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