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Article

HLA-DQB1 allele typing by a new PCR-RFLP method: correlation with a PCR-SSO method.

Tissue antigens - 1 Sept 1992

Salazar M, Yunis J J, Delgado M B, Bing D, Yunis E J

Abstract excerpt

We have developed a new PCR-RFLP method for HLA-DQB1 typing. This method was easy to follow, requiring only one DQB1 generic amplification and 5 endonucleases to assign 14 out of 15 HLA-DQB1 alleles. In addition, we determined that by using one generic amplification and two enzymes (Sau96 I and Hae III) it was possible to type the generic specificities: DQw2, DQw4, DQw5, DQw6, and DQw7, DQw8-9, providing a...

Topics

Alleles
Base Sequence
Deoxyribonucleases, Type II Site-Specific
Genes, MHC Class II
HLA-DQ Antigens
HLA-DQ beta-Chains
Histocompatibility Testing
Humans
Molecular Sequence Data
Oligonucleotide Probes
Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polymorphism, Restriction Fragment Length

Source

Identifiers

PMID: 1359671
DOI: 10.1111/j.1399-0039.1992.tb02102.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1359671-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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