Article
A mutation in codon 717 of the CHO-K1 elongation factor 2 gene prevents the first step in the biosynthesis of diphthamide.
Somatic cell and molecular genetics - 1 May 1992
Foley B T, Moehring J M, Moehring T J
Abstract excerpt
The histidine residue at position 715 of elongation factor 2 (EF-2) is posttranslationally modified in a series of enzymatic reactions to 2-[3-carboxyamido-3-(trimethylammonio)-propyl]histidine, which has been given the trivial name diphthamide. The diphthamide residue of EF-2 is the target site for ADP ribosylation by diphtheria toxin and Pseudomonas exotoxin A. ADP-ribosylated EF-2 does not function in protein...
Topics
- Adenine
- Amino Acid Sequence
- Animals
- Arginine
- Base Sequence
- CHO Cells
- Codon
- Cricetinae
- Glycine
- Guanine
- Histidine
- Homozygote