Article
Diphthamide modification on eukaryotic elongation factor 2 is needed to assure fidelity of mRNA translation and mouse development.
Proceedings of the National Academy of Sciences of the United States of America - 21 Aug 2012
Liu Shihui, Bachran Christopher, Gupta Pradeep, Miller-Randolph Sharmina, Wang Hailun, Crown Devorah, Zhang Yi, Wein Alexander N, Singh Rajat, Fattah Rasem, Leppla Stephen H
Abstract excerpt
To study the role of the diphthamide modification on eukaryotic elongation factor 2 (eEF2), we generated an eEF2 Gly(717)Arg mutant mouse, in which the first step of diphthamide biosynthesis is prevented. Interestingly, the Gly(717)-to-Arg mutation partially compensates the eEF2 functional loss resulting from diphthamide deficiency, possibly because the added +1 charge compensates for the loss of the +1 charge on...
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