Article
Characterization of the endogenous ADP-ribosylation of wild-type and mutant elongation factor 2 in eukaryotic cells.
European journal of biochemistry - 1 Apr 1992
Fendrick J L, Iglewski W J, Moehring J M, Moehring T J
Abstract excerpt
Anti-[ADP-ribosylated elongation factor 2 (EF-2)] antiserum has been used to immunoprecipitate the modified form of EF-2 from polyoma-virus-transformed baby hamster kidney (pyBHK) cells [Fendrick, J. L. & Iglewski, W. J. (1989) Proc. Natl Acad. Sci. USA 86, 554-557]. This antiserum also immunopre...
Topics
- Adenosine Diphosphate Ribose
- Animals
- CHO Cells
- Cell Line
- Cell Transformation, Viral
- Chromatography, High Pressure Liquid
- Chromatography, Thin Layer
- Cricetinae
- Diphtheria Toxin
- Electrophoresis, Polyacrylamide Gel
- Histidine
- Kinetics
- Mutation
- Peptide Elongation Factor 2
- Peptide Elongation Factors
- Phosphoric Diester Hydrolases
- Poly(ADP-ribose) Polymerases
- Precipitin Tests
