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Article

A dominant-negative approach that prevents diphthamide formation confers resistance to Pseudomonas exotoxin A and diphtheria toxin.

PloS one - 23 Dec 2010

Roy Vincent, Ghani Karim, Caruso Manuel

Abstract excerpt

Diphtheria toxin (DT), Pseudomonas aeruginosa Exotoxin A (ETA) and cholix toxin from Vibrio cholerae share the same mechanism of toxicity; these enzymes ADP-rybosylate elongation factor-2 (EF-2) on a modified histidine residue called diphthamide, leading to a block in protein synthesis. Mutant Chinese hamster ovary cells that are defective in the formation of diphthamide have no distinct phenotype except their...

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Identifiers

PMID: 21203470
DOI: 10.1371/journal.pone.0015753
PMCID: PMC3009735

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A dominant-negative approach that prevents diphthamide formation confers resistance to Pseudomonas exotoxin A and diphtheria toxin.PMID 21203470Read ↗

The biosynthesis and biological function of diphthamideSemantic proximity Read ↗

A mutation in codon 717 of the CHO-K1 elongation factor 2 gene prevents the first step in the biosynthesis of diphthamide.Semantic proximity Read ↗

Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2.Semantic proximity Read ↗

Insights into diphthamide, key diphtheria toxin effector.Semantic proximity Read ↗

Characterization of a Chinese hamster ovary cell mutant having a mutation in elongation factor-2.Semantic proximity Read ↗

The role of the diphthamide-containing loop within eukaryotic elongation factor 2 in ADP-ribosylation by Pseudomonas aeruginosa exotoxin A.Semantic proximity Read ↗

Dph3, a small protein required for diphthamide biosynthesis, is essential in mouse development.Semantic proximity Read ↗

Retroviral insertional mutagenesis identifies a small protein required for synthesis of diphthamide, the target of bacterial ADP-ribosylating toxins.Semantic proximity Read ↗

A Modified Form of Diphthamide Causes Immunotoxin Resistance in a Lymphoma Cell Line with a Deletion of the WDR85 GeneSemantic proximity Read ↗

Diphthamide modification of eEF2 requires a J-domain protein and is essential for normal development.Semantic proximity Read ↗

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Targeted introduction of a diphtheria toxin resistant mutation into the chromosomal EF-2 locus of Pichia pastoris and expression of immunotoxin in the EF-2 mutants.Semantic proximity Read ↗

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