Rapid and effective detection of mutations in the p53 gene using nonradioactive single-strand conformation polymorphism (SSCP) technique applied on PhastSystem.

doi:10.1016/0166-0934(94)00099-3

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Article

Rapid and effective detection of mutations in the p53 gene using nonradioactive single-strand conformation polymorphism (SSCP) technique applied on PhastSystem.

Journal of virological methods - 1 Jan 1995

Kurvinen K, Hietanen S, Syrjänen K, Syrjänen S

Abstract excerpt

The polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) method is a powerful tool for the screening of genetic alterations, including single-base substitutions. In the present study, the conventional SSCP technique was modified on the semiautomated electrophoresis system (PhastSystem) for the detection of mutations in the p53 tumor suppressor gene. The SSCP running conditions were...

Topics

Base Sequence
Cell Line
DNA
DNA Mutational Analysis
DNA Primers
DNA, Neoplasm
Evaluation Studies as Topic
Exons
Female
Genes, p53
Humans

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Nucleic Acid Conformation

Polymerase Chain Reaction

Polymorphism, Genetic

Tumor Cells, Cultured

Vaginal Neoplasms

Source

Open in PubMed

Identifiers

PMID: 7730436
DOI: 10.1016/0166-0934(94)00099-3

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7730436-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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