Article
Detection of ras gene mutations in human lung cancers by single-strand conformation polymorphism analysis of polymerase chain reaction products.
Oncogene - 1 Jul 1990
Suzuki Y, Orita M, Shiraishi M, Hayashi K, Sekiya T
Abstract excerpt
A simple, sensitive method of DNA analysis of nucleotide substitutions, namely, single-strand conformation polymorphism analysis of polymerase chain reaction products (PCR-SSCP analysis), was used for detection of mutated ras genes in surgical specimens of human lung cancer. Of a total of 129 tum...
Topics
- Base Sequence
- Carcinoma
- DNA, Neoplasm
- Electrophoresis, Agar Gel
- Genes, ras
- Humans
- Lung Neoplasms
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Oligonucleotides
- Polymerase Chain Reaction